The HPLC blood test expanded as a High Performance Liquid Chromatography test, is performed to screen Thalassemias and Hemoglobinopathies. This test is performed by taking the sample mixture and separating it for further diagnosis in a medical laboratory. You can benefit from complete HPCL test Delhi, which helps identify the abnormal hemoglobin, Hb A2, Hb F, etc.
HPLC test is quite quick to show the results with high levels of accuracy and reliability in the results. There are multiple laboratories that charge various amounts for HPCL test price. CNC Pathlab is a leading medical laboratory that is equipped with the latest technology required for proper HPCL blood test.
Some of the essential elements of chromatographs are high-performance pumps for delivering solvent at a constant flow rate and devices specifically designed for molecular separation called columns. The system formerly known as High Performance Liquid Chromatography was more often referred to as "LC '' as related technologies advanced. A high-speed analysis method known as Ultra High Performance Liquid Chromatography (UHPLC) has also grown in popularity in recent years.
The various forms of haemoglobin found in the blood are identified using haemoglobin (Hb) electrophoresis by HPLC. The red blood cells include the protein haemoglobin, which is responsible for carrying oxygen to the body's tissues and organs. This test is used to identify blood diseases, screen for hereditary problems, and assess how well blood disorder patients are responding to treatment. Additionally, it finds aberrant haemoglobin types that might obstruct the passage of oxygen.
Why is HPLC done?
As a component of a regular checkup
Identifying blood diseases
To keep track of the course of treatment for patients with abnormal haemoglobin
As a genetic disease screening test
What is measured by HPLC?
Different forms of haemoglobin in the blood are measured and identified by the haemoglobin (Hb) electrophoresis by HPLC test. Different forms of haemoglobin are found in the blood of humans. The amount of haemoglobin in the body changes with age. The protein found inside red blood cells is called haemoglobin. Its job is to carry oxygen to the body's tissues and organs.
The process of making haemoglobin is impacted when there is a mutation, a change in the DNA sequence. Hemoglobin exhibits anomalous behaviour as a result of these modifications, which also affects how normally healthy haemoglobin functions. As a result, less oxygen will need to be carried to tissues and organs.
This test uses a variety of haemoglobin types, including:
- Hemoglobin A - Adult hemoglobin
- Hemoglobin F
Newborns and developing foetuses both contain haemoglobin F. Hemoglobin A quickly takes its position after birth. Both adults and children have haemoglobin A. The most typical type of haemoglobin is that one. Rare aberrant haemoglobin types caused by genetic abnormalities include haemoglobin C, D, E, M, and S.
Types of HPLC columns
Normal Phase Columns
When the stationary phase is more polar than the mobile phase, normal phase columns are employed.
For instance, in normal phase chromatography, the less polar mobile phase might be hexane or similar organic solvent, and the polar stationary phase—also known as packing material—might be silica gel.
For samples containing tiny compounds, such as organic acids or medicines, this sort of column is frequently utilised. Biomolecules like glycosylated proteins can also employ it.
Reverse Phase Columns
When the stationary phase is less polar than the mobile phase, reverse phase columns are employed in reverse phase chromatography. To put it another way, reverse phase chromatography.
Acetonitrile, methanol, and tetrahydrofuran are frequent stationary phases, while water is frequently employed as the mobile phase (THF).
Compared to normal phase chromatography, this technique is more frequently utilised since it may be applied to a variety of analytical tasks.
Ion Exchange Columns
Ion exchange chromatography has a somewhat different method of column separation. This technique employs charge to separate chemicals that can be easily ionised rather than solely relying on polarity.
The mobile phase is often a polar aqueous buffer, such as salt water, while the stationary phase is typically an acid with either a positive or negative charge.
The attractive ionic interactions between the molecules in the sample and the charged stationary phase cause separation in this sort of column. As the sample goes through the column, the components will separate at various rates because each component will have a distinct level of attraction to the charged stationary phase.
Proteins, amino acids, and carbohydrates are typically separated using this technique.
Exclusion columns for sizes
Particle size is used in size exclusion chromatography to separate the material.
The stationary phase is porous, allowing only small particles to enter the pores while allowing bigger molecules to move through the column more quickly. Each molecule diffuses into the pores to a different amount, hence the size of the holes in the stationary phase controls the retention period and elution profile of each sample component.
For size exclusion chromatography, the stationary phase must have porous particles, which is why molecular sieves (like zeolites), polysaccharides, and polymers (like a conventional silica column) are most frequently utilised.
Proteins and carbohydrates are separated using this method.
We have an experienced certified, and well-trained staff that handles the entire process professionally and with complete precision. A blood sample is taken, which is further sent to the lab for evaluation and spotting the disease. We also offer online home sample collection services for the ease of our customers. At CNC Pathlab, you get to book full HPCL test Delhi at affordable rates. Our esteemed customer base is the testimony for our superior services.
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