NABL | ICMR Accredited Lab | ICMR Reg. No.: CNCPLDD
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Mismatch repair with reflex to MLH1 promoter methylation

Description- This test distinguishes sporadic from Lynch (HNPCC)-associated noncolorectal cancers with abnormal MLH1 Immunostaining. This test is preferred reflex screening test for Lynch syndrome in non-colorectal cancer tumors (such as endometrial carcinoma).

Methodology: Immunohistochemistry

 

TAT: 3-5 days

 

Specimen Preparation
Collect - tissue /or cell
Specimen Preparation- formal fix (10 % neutral buffered formalin) and paraffin enter specimen (FFPE specimens square measure needed). shield paraffin block or slides from uncontrolled heat. Transport tissue block or five unstained (3-micron thick sections), charged slides during a tissue transport kit. (Min: a pair of slides). If causing precut slides, don't over heat it.
Storage/Transport Temperature- At temperature or cold. Transport it in cooled instrumentation throughout weather.
Unacceptable conditions- i) Specimens submitted with non-marked/tagged tissue sort square measure rejected.
ii) Depleted specimens aren't tested.
Stability- i) Ambient: Indefinitely;
ii) Refrigerated: Indefinitely;
iii) Frozen: Unacceptable

 

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