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Mismatch repair with reflex to BRAF Codon 600 mutation

Description- This test distinguishes sporadic from Lynch (HNPCC)-associated colorectal cancers with abnormal MLH1 Immunostaining. This test is preferred screening test for Lynch syndrome (LS) in individuals with colorectal cancer. It provides definitive diagnosis of LS which requires additional targeted germline mismatch repair gene testing. Not preferred in case of endometrial cancer.

Methodology: Immunohistochemistry

 

TAT: 3-5 days

 

Specimen Preparation
Collect - tissue /or cell
Specimen Preparation- formal fix (10 % neutral buffered formalin) and paraffin enter specimen (FFPE specimens square measure needed). shield paraffin block or slides from uncontrolled heat. Transport tissue block or five unstained (3-micron thick sections), charged slides during a tissue transport kit. (Min: a pair of slides). If causing precut slides, don't over heat it.
Storage/Transport Temperature- At temperature or cold. Transport it in cooled instrumentation throughout weather.
Unacceptable conditions- i) Specimens submitted with non-marked/tagged tissue sort square measure rejected.
ii) Depleted specimens aren't tested.
Stability- i) Ambient: Indefinitely;
ii) Refrigerated: Indefinitely;
iii) Frozen: Unacceptable

 

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