MGMT by PCR TemozolomideResistance (MGMT Methylation
DESCRIPTION – it is strong predictive factor of favorable survival in glioblastoma multiform (GBM) patients undergoing chemotherapy with alkylating agents.
TEST CODE – ME1102
METHODOLOGY - methylation specific polymerase chain reaction
TURN AROUND TIME – 10 days
PATIENT PREPARATION –
COLLECT – tumor tissue
SPECIMEN PREPARATION – formalin fixed paraffin embedded tissue block. Protect the slides from excessive heat.
STORAGE/TRANSPORT TEMPERATURE – room temperature (8-25 degrees C)
STABILITY – ambient: indefinitely; refrigerated: indefinitely
UNACCEPTABLE CONDITIONS – alternate fixatives and decalcified specimens
MRP – 8500
SPECIAL INSTRUCTIONS – clinical history is mandatory
parafin embeded tissue block