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IDH1 R132H

Description- This test distinguishes primary from secondary glioblastoma multiform (GBM). IDH1 mutations occur in approximately 70% of astrocytomas and oligodendroglial tumors. This test allows the highly sensitive and specific demonstration of various tumors (such as astrocytoma from primary glioblastomas or diffuse astrocytoma grade II from pilocytic astrocytoma or ependymoma)

Methodology: Immunohistochemistry

 

TAT: 3-5 days

 

Specimen Preparation
Collect - tissue /or cell
Specimen Preparation- formal fix (10 % neutral buffered formalin) and paraffin enter specimen (FFPE specimens square measure needed). shield paraffin block or slides from uncontrolled heat. Transport tissue block or five unstained (3-micron thick sections), charged slides during a tissue transport kit. (Min: a pair of slides). If causing precut slides, don't over heat it.
Storage/Transport Temperature- At temperature or cold. Transport it in cooled instrumentation throughout weather.
Unacceptable conditions- i) Specimens submitted with non-marked/tagged tissue sort square measure rejected.
ii) Depleted specimens aren't tested.
Stability- i) Ambient: Indefinitely;
ii) Refrigerated: Indefinitely;
iii) Frozen: Unacceptable

 

 

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