BCR-ABLtranslocation [t(9:22)]

DESCRIPTION – BCR-ABL mutations are caused by the break off of both genes and then changes their places with each other. ABL gene is present on chromosome number 9 and BCR gene is present on chromosome number 22

CLINICAL UTILITY – it helps in the diagnosis of chronic myelogenous leukemia (CML) or a type of acute lymphoblastic leukemia (ALL)

METHODOLOGY – fluorescence in situ hybridization (FISH)

TURN AROUND TIME – 5 days

PATIENT PREPARATION –

COLLECT – non diluted bone marrow aspirate

SPECIMEN PREPARATION – 2-3 ml bone marrow or 3-4 ml peripheral blood in sodium heparin

STORAGE/TRANSPORT TEMPERATURE – room temperature

STABILITY – ambient: 48 hours, refrigerated: 48 hours

UNACCEPTABLE CONDITIONS – clotted specimens

MRP – 3000.00

SPECIAL INSTRUCTIONS – clinical history is mandatory