Trisomy 8

DECRIPTION – trisomy 8 is relatively specific for myeloid disorders and is rarely observed in lymphoid disease. It is found in 10-15% of patients with acute myeloid leukemia (AML), 15-20% of patients with myelodysplastic syndrome (MDS) as a secondary abnormality in Philadelphia chromosome positive CML and in other myeloproliferative disorders. Trisomy 8 occurs as the sole chromosome abnormality in about 40% of AML cases occurs in combination with simple chromosome occurs in 35% of cases and as part of complex karyotype in 35% of AML

METHODOLOGY – fluorescence in situ hybridization (FISH)

TURN AROUND TIME – 6 days

PATIENT PREPARATION –

COLLECT – non diluted bone marrow aspirate or sodium heparin

SPECIMEN PREPARATION – 3-4 ml of bone marrow or peripheral blood in green top (sodium heparin) or lavender top (EDTA) tube

STORAGE/TRANSPORT TEMPERATURE – 2-8 degrees C (with cold pack) and transport within 72 hours

STABILITY – ambient: 48 hours; refrigerated: 48 hours

UNACCEPTABLE CONDITIONS – frozen and clotted specimens

MRP – 3500.00

SPECIAL PREPARATION – clinical history is mandatory