Trisomy 12

DESCRIPTION – Trisomy 12 is the common cytogenetic abnormality and has various features including abnormal morphology and increased prevalence of NOTCH1 mutation

CLINICAL UTILITY – trisomy 12 is one of the most commonly reported chromosome aberration in B-cell chronic lymphocytic leukemia and is of prognostic importance. FISH analysis detects trisomy 12 in non-dividing cells which cannot be identified with routine cytogenetic studies

METHODOLOGY – fluorescence in situ hybridization (FISH)

TURN AROUND TIME – 7 days

PATEINT PREPARATION –

COLLECT – non diluted bone marrow aspirate or sodium heparin

SPECIMEN PREPARATION – 3-4 ml of bone marrow or peripheral blood in green top (sodium heparin) or lavender top (EDTA) tube

STORAGE/TRANSPORT TEMPERATURE – 2-8 degrees C (with cold pack) and transport within 72 hours

STABILITY – ambient: 48 hours; refrigerated: 48 hours

UNACCEPTABLE CONDITIONS – frozen or clotted specimens

MRP – 3500.00

SPECIAL INSTRUCTIONS – clinical history is mandatory