t(9;22): by Real time PCR Bcr-AblInternational Scale Blood

DESCRIPTION – the Philadelphia chromosome arising from the reciprocal t (9;22) translocation is found in more than 90% of chronic myeloid leukemia patients and results in the formation of the chimeric fusion gene BCR-ABL

METHODOLOGY – polymerase chain reaction (PCR)

TURN AROUND TIME – 4 days

PATIENT PREPARATION –

COLLECT – lavender (EDTA) or bone marrow or extracted RNA

SPECIMEN PREPARATION – transport 5 ml whole blood or 3 ml bone marrow. Specimens must be received within 48 hours of collection due to liability of RNA. Transport 40 micro-liter RNA

STORAGE/TRANSPORT TEMPERATURE – whole blood or bone marrow should be critically refrigerated and extracted RNA should be critically frozen

STABILITY – ambient: 1 hour; refrigerated: 48 hours; frozen: indefinitely (extracted RNA)

UNACCEPTABLE CONDITION – serum, plasma, CSF, extracted DNA, clotted specimens

MRP – 5500.00

SPECIAL INSTRUCTIONS – clinical history is mandatory