NRAS mutations Exon-1 & 2 Tissue

DESCRIPTION – determination of NRAS status plays a role in determining the outcome of therapy in colorectal carcinoma. Absence of NRAS mutation within the tumor suggests that the patient may respond to the therapy but other mutation such as EGFR, KRAS etc. also needs to be checked prior to NRAS

TEST CODE – ME1069

METHODOLOGY – real time polymerase chain reaction (real time PCR)

TURN AROUND TIME – 8 days

PATIENT PREPARATION –

COLLECT – tumor tissue

SPECIMEN PREPARATION – formalin fixed paraffin embedded tissue block. Protect the slides from excessive heat.

STORAGE/TRANSPORT TEMPERATURE – room temperature (8-25 degrees C)

STABILITY – ambient: indefinitely; refrigerated: indefinitely

UNACCEPTABLE CONDITIONS – alternate fixatives and decalcified specimens

MRP – 6000

SPECIAL INSTRUCTIONS – clinical history is mandatory