MGMT by PCR TemozolomideResistance (MGMT Methylation

DESCRIPTION – it is strong predictive factor of favorable survival in glioblastoma multiform (GBM) patients undergoing chemotherapy with alkylating agents.

TEST CODE – ME1102

METHODOLOGY - methylation specific polymerase chain reaction

TURN AROUND TIME – 10 days

PATIENT PREPARATION –

COLLECT – tumor tissue

SPECIMEN PREPARATION – formalin fixed paraffin embedded tissue block. Protect the slides from excessive heat.

STORAGE/TRANSPORT TEMPERATURE – room temperature (8-25 degrees C)

STABILITY – ambient: indefinitely; refrigerated: indefinitely

UNACCEPTABLE CONDITIONS – alternate fixatives and decalcified specimens

MRP – 8500

SPECIAL INSTRUCTIONS – clinical history is mandatory