What is a CD14 test?

A CD14 test is a diagnostic test that measures the levels of the CD14 protein in the blood. CD14 is a protein found on the surface of certain cells, including immune cells such as monocytes and macrophages. The CD14 test is often used to help diagnose infections or inflammatory conditions, as elevated levels of CD14 can indicate activation of the immune system. The test may also be used to monitor the progress of certain diseases or to assess the effectiveness of treatment. Overall, the CD14 test can provide valuable information for healthcare providers in diagnosing and managing a variety of health conditions.

What is the purpose of a CD14 test?

A CD14 test is a diagnostic test used to measure the level of CD14 protein in the blood. CD14 is a protein found on the surface of certain immune cells, including monocytes and macrophages. The purpose of this test is to help diagnose bacterial infections and monitor the effectiveness of treatment. When bacterial infections are present, the level of CD14 increases in response to the infection. Therefore, a high CD14 level can indicate the presence of a bacterial infection. This test is commonly used in hospital settings and can be ordered by a doctor or healthcare provider.

What type of sample is required for a CD14 test?

A CD14 test typically requires a blood sample. This test measures the levels of CD14, a receptor protein found on the surface of certain immune cells, in the blood. Elevated levels of CD14 have been associated with various inflammatory and infectious conditions, making the test useful for diagnostic purposes. It is important to consult with a healthcare provider for proper testing and interpretation of results.

How is a CD14 test performed?

A CD14 test is a diagnostic test that measures the levels of CD14, a protein found in certain immune cells, in a person's blood. This test is commonly used to diagnose bacterial infections and monitor the effectiveness of treatment.

To perform a CD14 test, a healthcare provider will typically draw a small amount of blood from the patient's arm using a needle. The blood sample is then sent to a laboratory for analysis.

In the laboratory, the blood sample is processed to isolate the immune cells and extract the CD14 protein. The CD14 protein is then measured using a technique called ELISA, which stands for enzyme-linked immunosorbent assay.

ELISA involves adding a specific antibody to the CD14 protein in the blood sample. The antibody binds to the CD14 protein, forming a complex that can be detected and measured using a special chemical reaction.

The results of the CD14 test are usually reported as a numerical value, which indicates the level of CD14 protein in the patient's blood. Higher levels of CD14 are typically associated with bacterial infections and other inflammatory conditions.

Overall, the CD14 test is a relatively simple and non-invasive diagnostic test that can provide valuable information about a patient's immune system function and overall health.

Is there any specialist recommendation required to get a CD14 test done in ?

Yes, a specialist recommendation may be required to get a CD14 test done in . It is best to consult with a doctor or a specialist such as a hematologist or immunologist who can assess your medical history and symptoms to determine if a CD14 test is necessary. Additionally, they may refer you to a diagnostic center or laboratory that can conduct the test. It is important to follow the guidance of your healthcare provider to ensure accurate testing and proper interpretation of results.

Is there any specific preparation required before getting a CD14 test done in ?

Before getting a CD14 test done in , there are a few preparations that one needs to make. It is advised to consult a doctor beforehand who can guide you about the test and its preparations. Some general preparations for the CD14 test may include:

1. Fasting: It is recommended to fast for 8-12 hours before undergoing the test. This means you should not eat or drink anything except water during this period.
2. Medications: If you are taking any medications, you should inform your doctor about them. Certain medications can interfere with the test and may need to be stopped before the test.
3. Inform the laboratory: Informing the laboratory about any medical conditions or allergies is important. This will help them take necessary precautions during the test.
4. Dress comfortably: You should wear comfortable clothes on the day of the test as it may take some time to complete the test.
5. Avoid strenuous exercise: It is advised to avoid any strenuous exercise or physical activity before the test as it may affect the results.

Overall, it is important to follow all instructions given by your doctor or healthcare provider for the CD14 test to ensure accurate results.

Description- CD14 acts as a co-receptor in detection of bacterial LPS that is expressed on cells of myelo-monocytic llineage, including monocytes, macrophages, and Langerhans cells

Methodology: Immunohistochemistry

TAT: 3-5 days

Specimen Preparation
Collect - tissue /or cell
Specimen Preparation- formal fix (10 % neutral buffered formalin) and paraffin enter specimen (FFPE specimens square measure needed). shield paraffin block or slides from uncontrolled heat. Transport tissue block or five unstained (3-micron thick sections), charged slides during a tissue transport kit. (Min: a pair of slides). If causing precut slides, don't over heat it.
Storage/Transport Temperature- At temperature or cold. Transport it in cooled instrumentation throughout weather.
Unacceptable conditions- i) Specimens submitted with non-marked/tagged tissue sort square measure rejected.
ii) Depleted specimens aren't tested.
Stability- i) Ambient: Indefinitely;
ii) Refrigerated: Indefinitely;
iii) Frozen: Unacceptable