10x Genomics Visium HD
Spatial Gene Expression
Map whole-transcriptome gene expression directly onto intact tissue architecture at near single-cell resolution.
Visium HD Spatial Gene Expression profiling reveals where genes are active within the tissue itself, preserving structural context that dissociation-based methods leave behind — built for oncology, neuroscience, immunology, and translational research programs.
What is Visium HD?
Visium HD is a high-definition spatial gene expression platform that reads transcripts directly from an intact tissue section, mapping each measurement back to the precise location it came from within the tissue.
Conventional RNA sequencing typically requires dissociating tissue into individual cells or processing bulk homogenate, which discards the spatial relationships between them. Spatial gene expression instead keeps the tissue section whole throughout the workflow, capturing whole-transcriptome data across a continuous, high-density grid so expression can be resolved at a scale approaching individual cells.
Because tissue architecture is preserved, researchers can overlay gene expression directly onto histology imagery — revealing how activity varies across cell neighborhoods, tissue compartments, and structural boundaries that would otherwise be invisible to dissociation-based methods.
- Maps whole-transcriptome expression to precise coordinates within the tissue.
- Preserves native tissue architecture throughout the entire workflow.
- Resolves expression differences at a scale approaching single cells.
- Overlays expression data directly onto histology imagery for intuitive review.
- Supports both fresh-frozen and FFPE tissue sections.
Key Benefits
A next-generation spatial platform built for depth, resolution, and clarity.
Ultra-High Spatial Resolution
Continuous, high-density capture areas resolve gene expression at a scale that approaches individual cells within intact tissue.
Whole-Transcriptome Profiling
Capture the full transcriptome from every tissue section instead of a pre-selected panel of targets.
Single-Cell Level Insights
Fine-grained bin sizing reveals expression differences between neighboring cells within the same tissue compartment.
High Sensitivity
Optimized chemistry captures low-abundance transcripts alongside highly expressed genes with confidence.
Preserved Tissue Morphology
The tissue section remains intact throughout the workflow, keeping structural landmarks fully visible.
Comprehensive Gene Expression Analysis
Full transcriptomic coverage supports discovery-driven exploration alongside hypothesis-led questions.
Advanced Visualization
Expression data overlays directly onto histology imagery for intuitive interpretation of spatial patterns.
Research-Ready Data
Structured outputs and annotated files are ready to drop into established spatial analysis pipelines.
Applications Across Research Domains
Spatial context supports discovery and translational goals across a broad range of tissue-based research.
Oncology Research
Map tumor heterogeneity, immune infiltration, and invasive margins directly within the tissue context.
Neuroscience
Resolve gene expression across cortical layers, nuclei, and cellular neighborhoods in neural tissue.
Immunology
Characterize immune cell organization and signaling niches within lymphoid and inflamed tissues.
Developmental Biology
Track spatial gene expression programs that guide tissue patterning across developmental stages.
Biomarker Discovery
Localize candidate biomarkers to specific tissue regions to strengthen confidence before validation.
Drug Discovery
Assess spatial response to treatment and identify tissue-level mechanisms of drug action.
Translational Research
Bridge molecular findings and tissue pathology to support translational study designs.
Precision Medicine
Build spatially resolved expression profiles that inform individualized research strategies.
A Guided Spatial Gene Expression Workflow
From tissue preparation to final data delivery, every step is tracked and quality-controlled.
Tissue Preparation
Fresh-frozen or FFPE tissue is processed and sectioned under strict quality-controlled conditions.
Tissue Section Placement
Sections are mounted onto the capture area, preserving native spatial orientation.
Histological Imaging
High-resolution brightfield or fluorescence imaging captures the tissue morphology for downstream overlay.
RNA Capture
Transcripts are captured directly from the tissue section across a continuous, high-density array.
Library Preparation
Captured transcripts are converted into sequencing-ready libraries under optimized chemistry.
Sequencing
Libraries are sequenced at depth sufficient to resolve whole-transcriptome spatial expression.
Bioinformatics Analysis
Reads are aligned, binned, and quantified against their originating spatial coordinates.
Spatial Visualization
Expression data is mapped back onto the tissue image for exploration and interpretation.
Final Data Delivery
Complete datasets, images, and reports are delivered through your secure client portal.
What You Receive
Complete, well-organized deliverables designed for downstream spatial analysis.
Raw Sequencing Data
Unprocessed reads provided for full transparency and independent reanalysis.
Spatial Gene Expression Matrix
Binned expression counts mapped to their precise spatial coordinates within the tissue.
High-Resolution Tissue Images
Full-resolution histology imagery aligned to the spatial expression data.
Quality Control Report
Coverage, capture efficiency, and imaging metrics summarized in a clear, reviewable format.
Bioinformatics Analysis
Processed clustering, spatial pattern, and differential expression results for your tissue.
Interactive Visualization Files
Ready-to-explore visualization outputs for reviewing spatial expression alongside tissue morphology.
Why Choose Our Laboratory
A trusted partner for spatial gene expression projects of every scale.
Certified Genomics Experts
A scientific team experienced in spatial transcriptomics study design and execution.
Advanced Sequencing Platforms
Modern instrumentation validated for high-depth, high-resolution spatial workflows.
Spatial Biology Expertise
Deep familiarity with tissue handling, imaging, and spatial data interpretation.
Reliable Quality Standards
Standardized protocols and multi-stage checkpoints protect data integrity at every step.
Fast Turnaround Time
Efficient pipelines built to keep pace with active research and publication timelines.
Dedicated Scientific Support
Responsive guidance available from project consultation through final data delivery.
Frequently Asked Questions
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