Calponin
Description- Calponin is used to characterize the differentiation process of mammary myoepithelial cells in the developing mammary gland and in investigation of the nature of myoepithelial cells also to study the development of human smooth muscle cells. Its high expression is also used as an additional marker of vascular smooth muscle cells, myopithelial cells in normal and benign human mammary gland and in certain stromal myofibroblasts
Methodology: Immunohistochemistry
TAT: 3-5 days
Specimen Preparation
Collect - tissue /or cell
Specimen Preparation- formal fix (10 % neutral buffered formalin) and paraffin enter specimen (FFPE specimens square measure needed). shield paraffin block or slides from uncontrolled heat. Transport tissue block or five unstained (3-micron thick sections), charged slides during a tissue transport kit. (Min: a pair of slides). If causing precut slides, don't over heat it.
Storage/Transport Temperature- At temperature or cold. Transport it in cooled instrumentation throughout weather.
Unacceptable conditions- i) Specimens submitted with non-marked/tagged tissue sort square measure rejected.
ii) Depleted specimens aren't tested.
Stability- i) Ambient: Indefinitely;
ii) Refrigerated: Indefinitely;
iii) Frozen: Unacceptable