T-cell Clonality Panel TCRG, TCRB Bone Marrow

DESCRIPTION – This assay employs multiple consensus DNA primers that target conserved genetic regions within the T cell receptor beta chain genes. This test is used to detect the vast majority of clonal T-cell malignancies from DNA

METHODOLOGY – capillary electrophoresis

TURN AROUND TIME – 5-9 days

SPECIMEN REQUIERED –

COLLECT – whole blood or bone marrow, formalin fixed tissue, lavender (EDTA), green (sodium heparin)

SPECIMEN PREPARATION – transport 5 ml whole blood, 3 ml bone marrow, 100 mg tissue (immediate freeze) or formalin fixed paraffin embedded tissue block

STORAGE/TRANSPORT TEMPERATURE –

Whole blood or bone marrow – refrigerated

Fresh tissue – frozen

FFPE tumor tissue – room temperature

STABILITY –

Whole blood – ambient: 24 hours; refrigerated: 5 days

Fresh tissue – refrigerated: 2 hours; frozen: 1 year

FFPE tumor tissue – ambient: indefinitely; refrigerated: indefinitely

UNACCEPTABLE CONDITION – plasma, serum, extracted DNA, clotted specimens, alternate fixatives

SPECIAL INSTRUCTIONS – clinical history is mandatory