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CD21

Description- CD21 is a C3D receptor, CR2 and EBV receptor which is expressed by follicular dendritic cells, mature B cells, some types of epithelial cells, reactive hyperplasia, and plasma cells. It has low expression on ALL cells, subset of normal thymocytes and mature T cells, lymphocytes in the mantle zone, sinus-lining cells, and monocytoid B cells. This aids in identifying B-cell CLL, follicular lymphoma, low-grade MALT-type B-cell lymphoma, primary salivary gland and gastric lymphoma, T-cell and histiocyte-rich B-cell lymphoma, angioimmunoblastic T-cell lymphoma, nodular lymphocyte- predominant Hodgkin lymphoma, follicular dendritic sarcoma, and some Reed-Sternberg cells not expressing other B- or T-cell-associated markers

Methodology: Immunohistochemistry

 

TAT: 3-5 days

 

Specimen Preparation
Collect - tissue /or cell
Specimen Preparation- formal fix (10 % neutral buffered formalin) and paraffin enter specimen (FFPE specimens square measure needed). shield paraffin block or slides from uncontrolled heat. Transport tissue block or five unstained (3-micron thick sections), charged slides during a tissue transport kit. (Min: a pair of slides). If causing precut slides, don't over heat it.
Storage/Transport Temperature- At temperature or cold. Transport it in cooled instrumentation throughout weather.
Unacceptable conditions- i) Specimens submitted with non-marked/tagged tissue sort square measure rejected.
ii) Depleted specimens aren't tested.
Stability- i) Ambient: Indefinitely;
ii) Refrigerated: Indefinitely;
iii) Frozen: Unacceptable

 

 

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